Updating the metagenomics toolbox
by the oxygen transfer capacity of the bioreactor, (ii) by the effect of dilution of the bioreactor content by the feed, i.e.
In both cases (a and b) the glucose concentration is very low after the initial batch phase.Currently however the industrial high cell-density processes rely on the fed-batch principle.In shaken cultures substrate-limited growth is not commonly applied as it is not trivial to implement a continuous well-controlled feed flow in a shaking environment.The biomass increase over the time is controlled either by the pump (a), or by the enzyme (b).
Many substrate limited fed-batch processes are started as a batch process, until the initial substrate, often glucose, is consumed.
all nutrients are added at the start of the cultivation).